Magnetically Alignable Bicelles with Unprecedented Stability Using Tunable Surfactants Derived from Cholic Acid

Ryoichi Matsui, Noriyuki Uchida, Masataka Ohtani, Kuniyo Yamada, Arisu Shigeta, Izuru Kawamura, Takuzo Aida, Yasuhiro Ishida

Abstract

Five novel surfactants were prepared by modifying the three hydroxy groups of sodium cholate with triethylene glycol chains endcapped with an amide (SC-C1, SC-nC4, and SC-nC5) or a carbamoyl group (SC-OnC4 and SC-OtC4). The phase behavior of aqueous mixtures of these surfactants with 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC) was systematically studied by 31PNMR spectroscopy. The surfactants endcapped with carbamate groups (SC-OnC4 and SC-OtC4) formed magnetically alignable bicelles over unprecedentedly wide ranges of conditions, in terms of temperature (from 21-23 to >90°C), lipid/surfactant ratio (from 5 to 8), total lipid content (5-20wt%), and lipid type [DMPC, 1,2-dilauroyl-sn-glycero-3-phosphatidylcholine (DLPC), or 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC)]. In conjunction with appropriate phospholipids, the carbamate-endcapped surfactants afforded unique bicelles, characterized by exceptional thermal stabilities (from 0 to >90°C), biomimetic lipid compositions (DMPC/POPC=25:75 to 50:50), and extremely large 2H quadrupole splittings (up to 71Hz).

Original languageEnglish
JournalChemPhysChem
DOIs
StateAccepted/In press - 2016

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Surface active agents
Lipids
Phospholipids
Biomimetics
Phase behavior
Glycols
Amides
Thermodynamic stability
Sodium
Spectroscopy
Acids
Chemical analysis
Temperature

Keywords

  • Bicelles
  • Cholic acid
  • Magnetic alignment
  • Phospholipids
  • Surfactants

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Physical and Theoretical Chemistry

Cite this

Magnetically Alignable Bicelles with Unprecedented Stability Using Tunable Surfactants Derived from Cholic Acid. / Matsui, Ryoichi; Uchida, Noriyuki; Ohtani, Masataka; Yamada, Kuniyo; Shigeta, Arisu; Kawamura, Izuru; Aida, Takuzo; Ishida, Yasuhiro.

In: ChemPhysChem, 2016.

Research output: Contribution to journalArticle

Matsui, Ryoichi; Uchida, Noriyuki; Ohtani, Masataka; Yamada, Kuniyo; Shigeta, Arisu; Kawamura, Izuru; Aida, Takuzo; Ishida, Yasuhiro / Magnetically Alignable Bicelles with Unprecedented Stability Using Tunable Surfactants Derived from Cholic Acid.

In: ChemPhysChem, 2016.

Research output: Contribution to journalArticle

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